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JoVE Journal Neuroscience
Kvantificering af subcellulære Ubiquitin-proteasome aktivitet i gnaver hjernen
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Kvantificering af subcellulære Ubiquitin-proteasome aktivitet i gnaver hjernen

Article DOI: 10.3791/59695-v • 09:25 min • May 21st, 2019
May 21st, 2019 •
1, 2, 1,2
1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 2School of Neuroscience, Virginia Polytechnic Institute and State University

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Denne protokol er designet til effektivt at kvantificere ubiquitin-proteasome system (UPS) aktivitet i forskellige cellulære rum i gnaver hjernen. Brugere er i stand til at undersøge UPS funktion i nukleare, cytoplasmatiske og synaptiske fraktioner i det samme dyr, hvilket reducerer mængden af tid og antal dyr, der er nødvendige for at udføre disse komplekse analyser.

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Neurovidenskab ubiquitin proteasome Nucleus synapse cytoplasma hukommelse hippocampus amygdala
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