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Journal / Neuroscience / Subcellular Ubiquitin ölçümü-kemirgen beyinde…
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JoVE Journal Neuroscience
Subcellular Ubiquitin ölçümü-kemirgen beyinde proteasom aktivitesi
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Subcellular Ubiquitin ölçümü-kemirgen beyinde proteasom aktivitesi

Article DOI: 10.3791/59695-v • 09:25 min • May 21st, 2019
May 21st, 2019 •
1, 2, 1,2
1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 2School of Neuroscience, Virginia Polytechnic Institute and State University

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Bu protokol, kemirgen beynin farklı hücresel bölmeler içinde Ubiquitin-proteasome sistemi (UPS) etkinliğini verimli bir şekilde ölçmek için tasarlanmıştır. Kullanıcılar, aynı hayvanlarda nükleer, sitoplazmik ve sinaptik fraksiyonlar içinde çalışan UPS 'i inceleyebilir, bu karmaşık analizleri gerçekleştirmek için gereken zaman ve hayvan sayısını azaltır.

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Nörobilim sayı 147 Ubiquitin proteasome çekirdek sinaps sitoplazma hafıza hipokampus amigdala
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