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Journal / Immunology and Infection / Kwantitatieve fluorescentie in situ-hybridisatie…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Kwantitatieve fluorescentie in situ-hybridisatie (vis) en immunofluorescentie (indien) van specifieke genproducten in KSHV-geïnfecteerde cellen
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Kwantitatieve fluorescentie in situ-hybridisatie (vis) en immunofluorescentie (indien) van specifieke genproducten in KSHV-geïnfecteerde cellen

Article DOI: 10.3791/59697-v • 06:21 min • August 27th, 2019
August 27th, 2019 •
1, 2
1Norfolk Academy, 2Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale School of Medicine

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We beschrijven een protocol met behulp van fluorescentie in situ hybridisatie (vissen) om meerdere herpes virale Rna's binnen lytisch geïnfecteerde menselijke cellen te visualiseren, hetzij in suspensie of aanhanger. Dit protocol omvat de kwantificering van fluorescentie die een nucleocytoplasmische verhouding produceert en kan worden uitgebreid voor gelijktijdige visualisatie van gastheer en virale eiwitten met immunofluorescentie (IF).

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Immunologie en infectie probleem 150 RNA vis indien herpesvirussen kwantificering nucleocytoplasmorische verschuiving suspensie cellen KSHV
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