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JoVE Journal
Immunology and Infection

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将鼠皮皮和皮米与热解辛分离,以检测部位特异性炎症mRNA和蛋白质
 
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将鼠皮皮和皮米与热解辛分离,以检测部位特异性炎症mRNA和蛋白质

Article DOI: 10.3791/59708-v 08:45 min September 29th, 2021
September 29th, 2021

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Summary

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这里介绍的是一个表皮与真皮分离的协议,用于评估炎症调解器的产生。炎症后,大鼠后爪表皮在4°C下由热利辛从真皮中分离出来。 然后,通过RT-PCR进行表皮分析,通过西方污点和免疫造血术进行蛋白质评估。

Tags

免疫学和感染, 第 175 期, 表皮, 热解质, 神经生长因子, 间柳金-6, 炎症, qPCR, 西印, 免疫造血术
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