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Journal / Immunology and Infection / Pan-lyssavirus Real Time RT-PCR for Rabies…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
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Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Article DOI: 10.3791/59709-v • 06:25 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1, 1, 1,2, 1, 1,2
1Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 2Institute of Infection and Global Health, University of Liverpool

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Summary
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This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.

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Pan-lyssavirus Real Time RT-PCR Rabies Diagnosis Ante-mortem Samples Post-mortem Samples Pan-Lyssavirus Primers Lyssavirus Genus Closed-tube Assay Rapid Diagnosis Sensitive Assay Decomposed Specimens Virus Culture Antigen Detection Methods Molecular Assays OIE Acceptance Good Laboratory Practice Contamination Risk Daisy Jennings Diagnostician RNA Quantification Micro Volume Spectrophotometer PCR Plate Layout PCR Workstation Preparation Reagent Thawing
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