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Journal / Immunology and Infection / Pan-lyssavirus in tempo reale RT-PCR per la…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Pan-lyssavirus in tempo reale RT-PCR per la diagnosi di rabbia
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Pan-lyssavirus in tempo reale RT-PCR per la diagnosi di rabbia

Article DOI: 10.3791/59709-v • 06:25 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1, 1, 1,2, 1, 1,2
1Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 2Institute of Infection and Global Health, University of Liverpool

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Questo RT-PCR in tempo reale utilizzando dsDNA tintura intercalating è adatto per diagnosticare le infezioni da lyssavirus. Il metodo inizia con l'RNA estratto dalla rabbia sospetti campioni ante mortem o post-mortem, dettagliando la preparazione del mix master, l'aggiunta di RNA, l'impostazione della macchina in tempo reale e la corretta interpretazione dei risultati.

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Immunologia e infezione Problema 149 Lyssavirus rabbia tempo reale RT-PCR diagnosi one-step
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