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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

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06:53 min

June 08, 2019

DOI:

06:53 min
June 08, 2019

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Summary

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Here, we present a protocol to detect tumor somatic mutations in circulating DNA present in patient biological fluids (biofluids). Our droplet digital polymerase chain reaction (dPCR)-based method enables quantification of the tumor mutation allelic frequency (MAF), facilitating a minimally invasive complement to diagnosis and temporal monitoring of tumor response.

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