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JoVE Journal
Cancer Research

This content is Open Access.

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
 

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Article doi: 10.3791/59721
June 8th, 2019 Usage Statistics

Summary June 8th, 2019

Here, we present a protocol to detect tumor somatic mutations in circulating DNA present in patient biological fluids (biofluids). Our droplet digital polymerase chain reaction (dPCR)-based method enables quantification of the tumor mutation allelic frequency (MAF), facilitating a minimally invasive complement to diagnosis and temporal monitoring of tumor response.

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