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Cancer Research

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JoVE Journal Cancer Research
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Article DOI: 10.3791/59721-v • 06:53 min • June 8th, 2019
June 8th, 2019 •
1,2, 1, 1,2, 3, 1, 4,5, 1,2,5
1Center for Genetic Medicine, Children's National Health System, 2Institute for Biomedical Sciences, The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology, University of California San Francisco, 5DIPG Centre of Expertise Zurich, Universitats-Kinderspital Zurich

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Summary
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Here, we present a protocol to detect tumor somatic mutations in circulating DNA present in patient biological fluids (biofluids). Our droplet digital polymerase chain reaction (dPCR)-based method enables quantification of the tumor mutation allelic frequency (MAF), facilitating a minimally invasive complement to diagnosis and temporal monitoring of tumor response.

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Detection Monitoring Tumor Associated Circulating DNA Patient Biofluids Tumor Mutations Invasive Surgical Procedures Tumor Mutation Load Tumor Genotyping Surgical Tissue Biopsy Challenging Locations Brain Stem Restricted Tissue Access Longitudinal Molecular Data MRI Digital PCR Monitoring Molecular Based Supplement Diagnosis Treatment Decision Making Process Gene Expression Fluo-4 And Quencher-linked Probes Quantitative PCR Droplet Digital PCR Procedure Bench Space Cleaning Equipment Cleaning Reagent Vortexing And Centrifugation Droplet Stabilizing Oil Nitrogen Gas Cylinder Attachment Pressure Setting Digital PCR Reaction Mixture
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