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Journal / Cancer Research / Detektion och övervakning av tumörassocierade…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Detektion och övervakning av tumörassocierade cirkulerande DNA i patientens Biofluider
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Journal (Cancer Research)
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Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
Journal (Cancer Research)
Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
 
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Detektion och övervakning av tumörassocierade cirkulerande DNA i patientens Biofluider

Article DOI: 10.3791/59721-v • 06:53 min • June 8th, 2019
June 8th, 2019 •
1,2, 1, 1,2, 3, 1, 4,5, 1,2,5
1Center for Genetic Medicine, Children's National Health System, 2Institute for Biomedical Sciences, The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology, University of California San Francisco, 5DIPG Centre of Expertise Zurich, Universitats-Kinderspital Zurich

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Här presenterar vi ett protokoll för att detektera tumör somatiska mutationer i cirkulerande DNA som finns i patientens biologiska vätskor (biofluider). Vår dropp Digital polymeras kedjereaktion (dpcr)-baserad metod möjliggör kvantifiering av tumör mutation alleliska frekvens (MAF), underlätta ett minimalt invasiva komplement till diagnos och tidsmässig övervakning av tumörrespons.

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Cancer forskning flytande biopsi digital dropp PCR cirkulerande cellfritt DNA plasma serum cerebrospinalvätska
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