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Journal / Biochemistry / En all-in-One-prøve holder til makromolekylær…
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Biochemistry

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JoVE Journal Biochemistry
En all-in-One-prøve holder til makromolekylær røntgenkrystallografi med minimal baggrunds spredning
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En all-in-One-prøve holder til makromolekylær røntgenkrystallografi med minimal baggrunds spredning

Article DOI: 10.3791/59722-v • 07:55 min • July 6th, 2019
July 6th, 2019 •
1, 2, 1
1Macromolecular Crystallography (HZB-MX), Helmholtz-Zentrum Berlin, 2Department Sample Environment, Helmholtz-Zentrum Berlin

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En roman prøveholder til makromolekylær røntgenkrystallografi sammen med en passende håndterings protokol præsenteres. Systemet giver krystalvækst, krystal iblødsætning og in situ diffraktion dataindsamling på både omgivende og kryogen temperatur uden behov for nogen krystal manipulation eller montering.

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Biokemi prøve indehaver in situ dataindsamling Cryo-beskyttelse ligand-iblødsætning krystalliserings platform hængende drop automatisering SPINE standard og makromolekylær krystallografi
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