Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biochemistry

This content is Open Access.

富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
 
Click here for the English version

富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定

Article DOI: 10.3791/59729-v 10:21 min June 20th, 2019
June 20th, 2019

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

用实例描述了含紫酯水酶的表达和纯化(大肠杆菌、FPLC中的表达)。纯化蛋白用于结晶和抗体生产,并用于酶测定。提出了选定的光度测定法,以显示FAHD1的多功能性,如牛酸脱碳酶和丙烯酰胺水酶。

Tags

生物化学 第148期 FAH FAHD FAHD1 ODx ApH 水酶 脱碳酰酶 牛乙酸酯 乙酰丙酸酯 紫霉素,丙烯酸盐 草酸盐 丙酸酯 米达斯
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter