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Journal / Biochemistry / Ekspression, rensning, krystallisering og enzym…
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Biochemistry

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JoVE Journal Biochemistry
Ekspression, rensning, krystallisering og enzym assays af Fumarylacetoacetat hydrolase-domæne-holdige proteiner
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Ekspression, rensning, krystallisering og enzym assays af Fumarylacetoacetat hydrolase-domæne-holdige proteiner

Article DOI: 10.3791/59729-v • 10:21 min • June 20th, 2019
June 20th, 2019 •
1,2, 1,2, 1,2, 1, 1, 3, 3, 4, 1,2
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria

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Udtryk og rensning af fumarylacetoacetat lav affinitet domæne-holdige proteiner er beskrevet med eksempler (udtryk i E. coli, fplc). Rensede proteiner bruges til krystallisering og antistof produktion og anvendes til enzym assays. Udvalgte fotometriske analyser er præsenteret for at vise multi-funktionaliteten af FAHD1 som oxaloacetat decarboxylase og acylpyruvate hydrolase.

Tags

Biokemi udstedelse 148 FAH FAHD FAHD1 ODx ApH hydrolase decarboxylase oxaloacetat acetylpyruvate fumarylpyruvate acylpyruvate oxalat pyruvate MiDAS
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