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Journal / Biochemistry / Uttryck, rening, kristallisering, och enzym…
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Biochemistry

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JoVE Journal Biochemistry
Uttryck, rening, kristallisering, och enzym analyser av Fumarylacetoacetat Hydrolase Domain-innehållande proteiner
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Journal (Biochemistry)
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
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Journal (Biochemistry)
Author Spotlight: Efficient Venom Extraction Method from Trichogramma Parasitoid Wasps (Video) | JoVE
 
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Uttryck, rening, kristallisering, och enzym analyser av Fumarylacetoacetat Hydrolase Domain-innehållande proteiner

Article DOI: 10.3791/59729-v • 10:21 min • June 20th, 2019
June 20th, 2019 •
1,2, 1,2, 1,2, 1, 1, 3, 3, 4, 1,2
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria

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Uttryck och rening av fumarylacetoacetat hydrolas domän-innehåll ande proteiner beskrivs med exempel (uttryck i E. coli, FPLC). Renade proteiner används för kristallisation och anti kropp produktion och användas för enzym analyser. Utvalda Foto metriska analyser presenteras för att Visa multifunktionaliteten hos FAHD1 som oxaloacetat dekarboxylas och acylpyruvate hydrolase.

Tags

Biokemi FAH FAHD FAHD1 ODx ApH hydrolase decarboxylase oxaloacetat acetylpyruvat fumarylpyruvat acylpyruvate oxalat Pyruvat MiDAS
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