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Journal / Biochemistry / Expressie, zuivering, kristallisatie, en enzym…
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Biochemistry

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JoVE Journal Biochemistry
Expressie, zuivering, kristallisatie, en enzym assays van Fumarylacetoacetate hydrolase domein-bevattende eiwitten
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Expressie, zuivering, kristallisatie, en enzym assays van Fumarylacetoacetate hydrolase domein-bevattende eiwitten

Article DOI: 10.3791/59729-v • 10:21 min • June 20th, 2019
June 20th, 2019 •
1,2, 1,2, 1,2, 1, 1, 3, 3, 4, 1,2
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria

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Expressie en zuivering van fumarylacetoacetate hydrolase domein-bevattende eiwitten wordt beschreven met voorbeelden (expressie in E. coli, FPLC). De gezuiverde proteïnen worden gebruikt voor kristallisatie en antilichamenproductie en in dienst genomen voor enzym analyses. De geselecteerde fotometrische analyses worden voorgesteld om de multi-functionaliteit van FAHD1 als Oxaloacetaat decarboxylase en acylpyruvate hydrolase te tonen.

Tags

Biochemie FAH FAHD FAHD1 ODx ApH hydrolase decarboxylase Oxaloacetaat acetylpyruvate fumarylpyruvate acylpyruvate oxalaat pyruvaat MiDAS
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