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Journal / Immunology and Infection / Eksperimentell analyse av apoptotisk Thymocyte…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Eksperimentell analyse av apoptotisk Thymocyte Oppslukningsprosess av makrofager
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Eksperimentell analyse av apoptotisk Thymocyte Oppslukningsprosess av makrofager

Article DOI: 10.3791/59731-v • 06:47 min • May 24th, 2019
May 24th, 2019 •
1, 1
1Division of Immunology, Allergy, and Rheumatology, Department of Internal Medicine, College of Medicine, University of Cincinnati

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Her presenterer vi en protokoll for å forberede apoptotisk thymocytes og bukhulen makrofager og analysere effektiviteten av efferocytosis og den spesifikke hemmer-mediert blokkering av apoptotisk thymocytes oppslukningsprosess. Denne protokollen har en bred anvendelse i celle-mediert klaring av andre partikler, inkludert kunstige perler og bakterier.

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Immunologi og infeksjon macrophage apoptotisk celler efferocytosis immunofluorescent mikroskopi Flow flowcytometri TAM reseptor tyrosin kinaser
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