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JoVE Journal Genetics
The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
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The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

Article DOI: 10.3791/59732-v • 08:12 min • June 12th, 2019
June 12th, 2019 •
1, 1
1Department of Biology, Villanova University

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The in vitro cell invasion assay is used to measure the potential of cancer metastasis by quantifying the cellular potential for invasion and migration using cell culture inserts containing protein matrix. Cells are challenged to migrate through the protein matrix and a porous membrane, towards a chemoattractant, and then quantified by light microscopy.

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Mouse Mammary Tumor Cells In Vitro Invasion Assay Oncogenic Cell Behavior Protein Rich Matrix Porous Membrane Cell Migration Cell Invasion Gene Expression Aggressive Cancers Cell Behavior Changes Genes And Factors Involved T25 Flask Adherent Cells Boyden Chamber Inserts Serum-free DMEM Media
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