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JoVE Journal Biochemistry
DNA-sekvens erkännande av DNA-Primase med hög genomströmning Primase profilering
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DNA-sekvens erkännande av DNA-Primase med hög genomströmning Primase profilering

Article DOI: 10.3791/59737-v • 08:04 min • October 8th, 2019
October 8th, 2019 •
1, 1, 2, 2, 1, 1
1Department of Chemistry, Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

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Proteinbindnings metoder för microarray (PBM) kombinerat med biokemiska analyser binder samman de bindande och katalytiska egenskaperna hos DNA-Primase, ett enzym som syntetiserar RNA-primers på mallDNA. Denna metod, som betecknas som hög genomströmning Primase profilering (HTPP), kan användas för att avslöja DNA-bindande mönster av en mängd olika enzymer.

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Biokemi hög genomströmning Primase profilering HTPP proteinbindning microarray PBM DNA-Primase DNA-replikering protein-DNA interaktion DNA-bindning RNA-primers molekylärbiologi
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