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JoVE Journal Developmental Biology
Entwicklung von Targeting Induzierte lokale Läsionen IN Genomen (TILLING) Populationen in Kleinkornkulturen durch Ethylmethansulfonat-Mutagenese
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Entwicklung von Targeting Induzierte lokale Läsionen IN Genomen (TILLING) Populationen in Kleinkornkulturen durch Ethylmethansulfonat-Mutagenese

Article DOI: 10.3791/59743-v • 08:36 min • July 16th, 2019
July 16th, 2019 •
1, 1, 1, 1, 1, 1, 1
1Department of Plant Science and Landscape Architecture, University of Maryland, College Park

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Described ist ein Protokoll zur Entwicklung einer Targeting Induced Local Lesions IN Genomes (TILLING) Population in Kleinkornkulturen mit Verwendung von Ethylmethansulfonat (EMS) als Mutagen. Ebenfalls zur Verfügung gestellt wird ein Protokoll zur Mutationsdetektion mit dem Cel-1-Assay.

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Entwicklungsbiologie Ausgabe 149 Mutagenese TILLING Population EMS Reverse Genetics Small Grains Cel-1 endonuclease
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