Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Mitochondria and Endoplasmic Reticulum Imaging by …
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets (Video) | JoVE
Journal (Biochemistry)
Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets (Video) | JoVE
On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
Journal (Biochemistry)
On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
 

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Article DOI: 10.3791/59750-v • 09:21 min • July 20th, 2019
July 20th, 2019 •
1, 1
1Neural Circuits Research Group, Korea Brain Research Institute

Chapters

Summary
Automatic Translation

We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.

Tags

Mitochondria Endoplasmic Reticulum Imaging Correlative Light Microscopy Volume Electron Microscopy Biological Studies 3D Structure Rod Fielder View Sample Storage Electron Test Target Structures Specific Target Of Interest Membranous Organelles Host Cells Transfection Fixation Solution Sodium Cacodylate Buffer Glycine Solution DAB Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter