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Journal / Biochemistry / Mitochondriën en Endoplasmisch reticulum…
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JoVE Journal Biochemistry
Mitochondriën en Endoplasmisch reticulum beeldvorming door Correlatief licht en volume elektronenmicroscopie
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Mitochondriën en Endoplasmisch reticulum beeldvorming door Correlatief licht en volume elektronenmicroscopie

Article DOI: 10.3791/59750-v • 09:21 min • July 20th, 2019
July 20th, 2019 •
1, 1
1Neural Circuits Research Group, Korea Brain Research Institute

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We presenteren een protocol voor het bestuderen van de verdeling van mitochondriën en endoplasmisch reticulum in hele cellen na genetische modificatie met behulp van correlatief licht en volume elektronenmicroscopie met inbegrip van ascorbaat peroxidase 2 en mierikswortelperoxidase kleuring, serie snijden van cellen met en zonder het doel gen in dezelfde sectie, en seriële beeldvorming via elektronenmicroscopie.

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Biochemie uitgave 149 mitochondriën ER APEX HRP correlatief licht en elektronenmicroscopie 3DEM
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