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Immunolabelling Myofiber degenerasjon i muskel biopsier
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Immunolabelling Myofiber degenerasjon i muskel biopsier

Article DOI: 10.3791/59754-v • 06:37 min • December 5th, 2019
December 5th, 2019 •
1,2, 1, 1, 1
1Inserm, Institut Mondor de Recherche Biomédicale, 2The Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Program, UCL Great Ormond Street Institute of Child Health

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Beskrevet her er en protokoll for direkte immunolabelling av nekrotisk myofibers i muskel cryosections. Nekrotisk celler er gjennomtrengelig for serumproteiner, inkludert immunglobulin G (IgG). Avslører opptaket av IgG ved myofibers tillater identifisering og kvantifisering av myofibers som gjennomgår nekrose uavhengig av muskel tilstand.

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Biologi muskel degenerasjon myofibers sarcolemma celle død muskel dystrofi nekrose myonecrosis necroptosis betennelse immunglobulin
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