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Journal / Genetics / Mätning av BK-polyomavirus icke-kodning kontroll…
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Genetics
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JoVE Journal Genetics
Mätning av BK-polyomavirus icke-kodning kontroll region driven transkriptionell aktivitet via flödescytometri
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Mätning av BK-polyomavirus icke-kodning kontroll region driven transkriptionell aktivitet via flödescytometri

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

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I detta manuskript, ett protokoll presenteras för att utföra FACS-baserade mätningar av BK-polyomavirus transkriptionell aktivitet genom att använda HEK293T celler transfekterade med en dubbelriktad reporter plasmid uttrycker tdtomato och egfp. Denna metod gör det möjligt att kvantitativt bestämma påverkan av nya föreningar på viral transkription.

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Genetik BK-polyomavirus BKPyV icke-kodning kontroll region NCCR dubbelriktad promotor aktivitet FACS antiviral aktivitet njurtransplantation stora T-antigen SV40
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