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Journal / Genetics / Misurazione di BK-poliomavirus Non-Coding Control …
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JoVE Journal Genetics
Misurazione di BK-poliomavirus Non-Coding Control Region Guidato Attività Trascrizionional Via Flusso Cytometry
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Misurazione di BK-poliomavirus Non-Coding Control Region Guidato Attività Trascrizionional Via Flusso Cytometry

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

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In questo manoscritto, viene presentato un protocollo per eseguire la misurazione basata su FACS dell'attività trascrizionale BK-poliomavirus utilizzando cellule HEK293T trascate da un plasmide reporter bidirezionale che esprime tdTomato ed eGFP. Questo metodo permette inoltre di determinare quantitativamente l'influenza di nuovi composti sulla trascrizione virale.

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Genetica Numero 149 BK-Polyomavirus BKPyV Regione di controllo non codificante NCCR attività promotrice bidirezionale FACS attività antivirale trapianto renale grande antigene T SV40
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