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Biochemistry

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使用含有微型基因的Alu元素来分析圆形RNA
 
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使用含有微型基因的Alu元素来分析圆形RNA

Article doi: 10.3791/59760
March 10th, 2020

Summary March 10th, 2020

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我们克隆和分析生成圆形RNA的记者基因。这些报告基因大于构造,用于分析线性拼接并包含Alu元素。为了研究圆形RNA,构造被转染到细胞中,在去除线性RNA后使用RT-PCR分析产生的RNA。

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