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JoVE Journal Biochemistry
Uso de Minigenes que contienen elementos Alu para analizar ARN circulares
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Uso de Minigenes que contienen elementos Alu para analizar ARN circulares

Article DOI: 10.3791/59760-v • 13:10 min • March 10th, 2020
March 10th, 2020 •
1, 1, 1, 1
1Institute for Molecular and Cellular Biochemistry, University of Kentucky

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Clonamos y analizamos genes de reportero generando ARN circulares. Estos genes reporteros son más grandes que las construcciones para analizar el empalme lineal y contener elementos Alu. Para investigar los ARN circulares, las construcciones se transinfectan en células y el ARN resultante se analiza utilizando RT-PCR después de la eliminación del ARN lineal.

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Bioquímica Número 157 ARN circulares empalme alternativo gen reportero minigen ARN ARNm
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