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JoVE Journal Neuroscience
Analyse der Spliceosomalen snRNA-Lokalisierung in menschlichen Hela-Zellen mit Mikroinjektion
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Analyse der Spliceosomalen snRNA-Lokalisierung in menschlichen Hela-Zellen mit Mikroinjektion

Article DOI: 10.3791/59797-v • 07:35 min • August 6th, 2019
August 6th, 2019 •
1,2, 1
1Laboratory of RNA Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, 2Department of Genetics and Microbiology, Faculty of Science, Charles University

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Biogenese von spliceosomals snRNAs ist ein komplexer Prozess mit verschiedenen Zellkompartimenten. Hier haben wir Mikroinjektionen von fluoreszierend gekennzeichneten snRNAs eingesetzt, um deren Transport innerhalb der Zelle zu überwachen.

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Neurowissenschaften Ausgabe 150 Mikroinjektion snRNA HeLa-Zellen Cajal-Körper Kern Zytoplasma
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