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Journal / Immunology and Infection / Enkelt celle screening metode til udvælgelse og…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Enkelt celle screening metode til udvælgelse og nyttiggørelse af antistoffer med ønskede karakteristika fra beriget menneskelig hukommelse B cellepopulationer
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Enkelt celle screening metode til udvælgelse og nyttiggørelse af antistoffer med ønskede karakteristika fra beriget menneskelig hukommelse B cellepopulationer

Article DOI: 10.3791/59809-v • 09:07 min • August 22nd, 2019
August 22nd, 2019 •
1, 1, 1, 2, 1
1World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

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BSelex metode til at identificere og genvinde individuelle antigen-specifikke antistoffer fra humant perifert blod mononukleære celler kombinerer flow cytometri med enkelt celle PCR og kloning.

Tags

Immunologi og infektion B celle repertoire B celle receptor enkelt celle kloning celle sortering antistof opdagelse B celle kloning hukommelse B celle tung kæde lyskæde
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