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Journal / Biochemistry / Real-Time, Semi-Automated Fluorescent Measurement …
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Biochemistry

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JoVE Journal Biochemistry
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
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Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

Article DOI: 10.3791/59815-v • 10:18 min • June 13th, 2019
June 13th, 2019 •
1, 2,3, 2, 2,4, 1,2, 2,3, 1
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co

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Summary
Automatic Translation

We present a protocol to make dynamic measurements of the airway surface liquid pH under thin film conditions using a plate-reader.

Tags

Real-time Measurement Fluorescent Measurement Airway Surface Liquid PH Human Airway Epithelial Cells Ion Channels Transporters Extracellular PH Regulation Cancer Cells Simple Technique No Specialized Equipment Multiple Cultures Air Liquid Interface Thin Film Conditions Therapeutics Assessment Cystic Fibrosis Chronic Airway Diseases Asthma COPD Culture Wash Bicarbonate Containing Krebs Buffer Solution Glass Pipet Aspiration Pump
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