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Inducerende og karakteriserende vesikulær steatosis i differentierede HepaRG-celler

Article DOI: 10.3791/59843-v • 09:15 min • July 18th, 2019
July 18th, 2019 •
1,2, 3, 3, 3, 2,4, 2,3,5,6, 1,3
1Department of Molecular Medicine, Sapienza University, 2Department of Internal Medicine - DMISM, Sapienza University, 3Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology, Croix Rousse Hospital, Hospices Civils de Lyon

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I denne undersøgelse beskriver vi en detaljeret protokol for at inducere levervesikulær steatose i differentierede heparg-celler med fedtsyresalt natriumoleat og anvende metoder til påvisning og kvantificering af lipid-akkumulering, herunder sammenhængende anti-Stokes Raman spredning (biler) mikroskopi, cytofluorimetriske analyse, olie rød O farvning, og qPCR.

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Medicin HepaRG lever vesikulær steatosis NAFLD lipid dråber biler mikroskopi
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