Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Indurre e caratterizzare la vesapica steatosi…
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.

JoVE Journal Medicine
Indurre e caratterizzare la vesapica steatosi nelle celle HepaRG differenziate
Suspension-Trapping Sample Preparation of Human Tear Protein (Video) | JoVE
Journal (Medicine)
Suspension-Trapping Sample Preparation of Human Tear Protein (Video) | JoVE
Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
Journal (Medicine)
Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
 
Click here for the English version

Indurre e caratterizzare la vesapica steatosi nelle celle HepaRG differenziate

Article DOI: 10.3791/59843-v • 09:15 min • July 18th, 2019
July 18th, 2019 •
1,2, 3, 3, 3, 2,4, 2,3,5,6, 1,3
1Department of Molecular Medicine, Sapienza University, 2Department of Internal Medicine - DMISM, Sapienza University, 3Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology, Croix Rousse Hospital, Hospices Civils de Lyon

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

In questo studio, descriviamo un protocollo dettagliato per indurre la steatosi vescicolare del fegato in cellule HepaRG differenziate con l'oleate di sodio acido grasso e impieghiamo metodi per il rilevamento e la quantificazione dell'accumulo di lipidi, compresi gli anti-Stokes coerenti Microscopia a dispersione Raman (CARS), analisi citofluorimetrica, colorazione Oo rosso olio e qPCR.

Tags

Medicina Numero 149 HepaRG fegato steatosi vescicolare NAFLD goccioline di lipidi microscopia CARS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter