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Cultures à court terme de tranches flottantes du cerveau humain adulte
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Cultures à court terme de tranches flottantes du cerveau humain adulte

Article DOI: 10.3791/59845-v • 09:14 min • November 5th, 2019
November 5th, 2019 •
1,2, 1,3, 1, 1, 4, 4, 5, 2, 3, 1
1Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, 2Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, 3Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, 4Department of Anatomy, Institute of Biosciences, São Paulo State University, 5Clinical Hospital at the Ribeirão Preto Medical School, University of São Paulo

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Un protocole pour préparer les cultures de tranches flottantes à partir du cerveau humain adulte est présenté. Le protocole est une variante de la méthode de culture de tranches largement utilisée à l'aide d'inserts membranaires. Il est simple, rentable et recommandé pour les essais à court terme visant à démêler les mécanismes de neurodégénérescence derrière les maladies du cerveau associées à l'âge.

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Neurosciences Numéro 153 histoculture néocortex ex vivo neurodégénérescence épilepsie Alzheimer
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