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Journal / Genetics / Direkter Gene Knock-out von Axolotl Spinal Cord…
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JoVE Journal Genetics
Direkter Gene Knock-out von Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
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Direkter Gene Knock-out von Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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Hier wird ein Protokoll zur Durchführung zeit- und raumbeschränkter Gen-Knock-out in Axolotl-Rückenmarksen vorgestellt, indem der CAS9-gRNA-Komplex in den Zentralkanal des Rückenmarks injiziert wird, gefolgt von Elektroporation.

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Genetik Ausgabe 149 Axolotl Rückenmark Regeneration CRISPR-Cas9 neuronale Stammzellen Gen-Knock-out
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