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Journal / Genetics / Direkte gen knock-out af Axolotl rygmarv neurale…
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Genetics

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JoVE Journal Genetics
Direkte gen knock-out af Axolotl rygmarv neurale stamceller via elektroporation af CAS9 protein-gRNA komplekser
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Direkte gen knock-out af Axolotl rygmarv neurale stamceller via elektroporation af CAS9 protein-gRNA komplekser

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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Præsenteret her er en protokol til at udføre tid-og rum-begrænset gen knock-out i Axolotler rygmarvs ledninger ved at injicere CAS9-gRNA kompleks i rygmarven central kanalen efterfulgt af elektro poration.

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Genetik Axolotl rygmarv regenerering CRISPR-Cas9 neurale stamceller gen knock-out
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