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Immunology and Infection

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JoVE Journal Immunology and Infection
Intravital Imaging av intraepithelial-lymfocytter i murine tynntarmen
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Intravital Imaging av intraepithelial-lymfocytter i murine tynntarmen

Article DOI: 10.3791/59853-v • 08:00 min • June 24th, 2019
June 24th, 2019 •
1, 1
1Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine, Rutgers New Jersey Medical School

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Vi beskriver en metode for å visualisere GFP-merket γδ IELs bruker intravital Imaging av murine tynntarmen av invertert spinning disk konfokalmikroskopi mikroskopi. Denne teknikken gjør det mulig sporing av levende celler i mucosa for opptil 4 h og kan brukes til å undersøke en rekke intestinal immun-epitel interaksjoner.

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Immunologi og infeksjon immunologi T celle intraepithelial lymfocytter mus tynntarmen intravital Imaging spinning disk konfokalmikroskopi mikroskopi celle sporing
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