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Ultralyd-forstærket primær voksen fibroblast isolation
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Ultralyd-forstærket primær voksen fibroblast isolation

Article DOI: 10.3791/59858-v • 06:51 min • July 29th, 2019
July 29th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1
1Institute of Pharmacology and Toxicology, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden

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Vi præsenterer en protokol for at isolere primære voksne fibroblaster på en nem, hurtig og pålidelig måde, performable af begyndere (f. eks. studerende). Proceduren kombinerer enzymatisk vævs nedbrydning og mekanisk agitation med ultralydbølger for at opnå primære fibroblaster. Protokollen kan let tilpasses specifikke eksperimentelle krav (f. eks. humant væv).

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Medicin fibroblaster fibrose primære celler cellekultur ultralyd celle isolation
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