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Ultrasoon-verhoogde primaire volwassen fibroblast-isolatie
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Ultrasoon-verhoogde primaire volwassen fibroblast-isolatie

Article DOI: 10.3791/59858-v • 06:51 min • July 29th, 2019
July 29th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1
1Institute of Pharmacology and Toxicology, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden

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We presenteren een protocol om primaire volwassen fibroblasten op een eenvoudige, snelle en betrouwbare manier te isoleren, die door beginners kunnen worden perforeerbaar (bijv. studenten). De procedure combineert enzymatische weefsel spijsvertering en mechanische opwinding met ultrasone golven om primaire fibroblasten te verkrijgen. Het protocol kan gemakkelijk worden aangepast aan specifieke experimentele eisen (bijv. menselijk weefsel).

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Geneeskunde probleem 149 fibroblasten fibrose primaire cellen celkweek ultrasoon celisolatie
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