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JoVE Journal Genetics
A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Article DOI: 10.3791/59864-v • 06:34 min • January 21st, 2020
January 21st, 2020 •
*1,2, *1,2, 2, 1
1Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, 2State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University
* These authors contributed equally

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Summary
Automatic Translation

A bioinformatics pipeline, namely miRDeep-P2 (miRDP2 for short), with updated plant miRNA criteria and an overhauled algorithm, could accurately and efficiently analyze microRNA transcriptomes in plants, especially for species with complex and large genomes.

Tags

Bioinformatics Pipeline MicroRNA Transcriptomes Plants MiRDeep2 Accurate Analysis Efficient Analysis Transcriptional Regulators Plant Development Environmental Challenges Running Time Sensitivity Accuracy False Positives Processing Time Fielding Strategy Scoring Algorithm Stringent Criteria MicroRNA Annotation Genome Function Step-by-step Guidance First-time Annotators Experienced Researchers Advantages Of MiRDeep2 Installation Instructions Test Data
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