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Journal / Biochemistry / Identifiering av inositol fosfat eller…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Identifiering av inositol fosfat eller fosfoinositid samverkande proteiner genom affinitetskromatografi kopplad till Western blot eller masspektrometri
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Identifiering av inositol fosfat eller fosfoinositid samverkande proteiner genom affinitetskromatografi kopplad till Western blot eller masspektrometri

Article DOI: 10.3791/59865-v • 08:07 min • July 26th, 2019
July 26th, 2019 •
1
1Institute of Parasitology, McGill University

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Detta protokoll fokuserar på identifiering av proteiner som binder till inositol fosfater eller phosphoinositides. Den använder affinitetskromatografi med biotinylerade inositol fosfater eller phosphoinositides som immobiliseras via konjugerat till aguppstod eller magnetiska pärlor. Inositol fosfat eller fosfoinositid bindande proteiner identifieras genom Western blotting eller masspektrometri.

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Denna månad i JoVE inositol fosfater fosfatidylinositol fosfoinositid protein interaktion Trypanosoma metaboliter masspektrometri Western blotting affinitetskromatografi
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