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Immunology and Infection
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Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

DOI:
10.3791/59868-v

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08:03 min

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July 15, 2019

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,
1Department of Molecular Genetics, Biochemistry, and Microbiology,University of Cincinnati College of Medicine

Chapters

  • 00:04Title
  • 00:54Tissue Digestion and Well Coating with BMM (Basement Membrane Matrix)
  • 02:07Filtering of Undigested Tissue, RBC Lysis, and Cell Plating
  • 03:53Subculturing the Salispheres and Maintenance on BMM
  • 05:23Subculturing the Salispheres on Treated Plastic Tissue Culture Dishes
  • 06:43Results: Primary Human Salisphere Cells Cultured as Spheres or as a Monolayer
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method for isolating and cultivating primary human salivary gland-derived epithelial cells. These cells exhibit gene expression patterns consistent with them being of salivary epithelial origin and can be grown as salispheres on basement membrane matrices derived from Engelbreth-Holm-Swarm tumor cells or as monolayers on treated culture dishes.

Tags

Keywords: Salivary Epithelial CellsPrimary Cell CultureSalispheresOrganoidSalivary GlandDispaseCollagenaseCell IsolationCell CultureCell Strainer

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