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JoVE Journal
Immunology and Infection

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从人类唾液腺中分离唾液表皮细胞,作为脂质层或单层体进行体外生长
 
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从人类唾液腺中分离唾液表皮细胞,作为脂质层或单层体进行体外生长

Article DOI: 10.3791/59868-v 08:03 min July 15th, 2019
July 15th, 2019

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Summary

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我们提出了一种分离和培养人类原发性唾液腺衍生上皮细胞的方法。这些细胞表现出与唾液上皮起源一致的基因表达模式,可以在从恩格尔布雷斯-霍尔姆-肖温肿瘤细胞中提取的基底膜基质上作为盐层生长,或作为治疗培养皿上的单层细胞生长。

Tags

免疫学和感染,第149期,唾液腺,原生培养物,盐层,器官,Sjögren综合征,人类巨细胞病毒,病毒复制模型,唾液
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