Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Jia-huan Lu; Yun Wang; Qi Meng; Zhao-lei Zeng

doi:10.3791/59871

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

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08:42 min

July 19, 2019

DOI: 10.3791/59871-v

Jia-huan Lu*¹, Yun Wang*¹, Qi Meng*¹, Zhao-lei Zeng¹

¹State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine,Sun Yat-sen University Cancer Center

Overview

This protocol outlines the establishment of patient-derived xenograft (PDX) models and primary cancer cell lines from surgical gastric cancer samples. These methods are essential for advancing drug development and cancer biology research.

Key Study Components

Area of Science

Cancer Biology
Drug Development
Xenograft Models

Background

Patient-derived xenograft models preserve the histologic appearance of cancer cells.
These models retain intratumoral heterogeneity.
They better reflect the human components of the tumor microenvironment.
PDX models are more accurate than traditional cancer cell lines.

Purpose of Study

To develop PDX models for improved preclinical evaluation of anticancer therapies.
To compare molecular characteristics between different cancer patient subgroups.
To enhance understanding of tumor biology and drug responses.

Methods Used

Establishment of PDX models from surgical gastric cancer samples.
Use of NSG mice as the immunodeficient mouse model.
Maintenance of sterility during experiments.
Analysis of tumor signatures and molecular characteristics.

Main Results

PDX models accurately represent human cancer biology.
These models show potential for improved drug testing.
They allow for the study of intratumoral heterogeneity.
Facilitate comparisons of tumor characteristics across patient subgroups.

Conclusions

PDX models are a valuable tool in cancer research.
They provide insights into tumor biology and treatment responses.
These models can lead to better therapeutic strategies for cancer patients.

Frequently Asked Questions

What are patient-derived xenograft models?

Patient-derived xenograft models are experimental systems that involve implanting human tumor tissues into immunocompromised mice to study cancer biology and treatment responses.

Why are NSG mice used in this research?

NSG mice are used due to their severe immunodeficiency, which allows for the growth of human tumors without rejection.

What is the significance of maintaining sterility in experiments?

Maintaining sterility is crucial to prevent contamination and ensure the validity of the experimental results.

How do PDX models compare to traditional cancer cell lines?

PDX models better preserve the complexity and heterogeneity of human tumors, making them more representative than traditional cancer cell lines.

What applications do PDX models have in cancer research?

PDX models are used for drug testing, understanding tumor biology, and comparing molecular characteristics among different cancer patient subgroups.

The current protocol describes methods to establish patient-derived xenograft (PDX) models and primary cancer cell lines from surgical gastric cancer samples. The methods provide a useful tool for drug development and cancer biology research.

Patient-derived Xenograft Models and Primary Cell Lines are becoming an integral part of drug development and the initiation and progression of tumor biology research. Patient-derived Xenograft Models preserve the histologic appearance of cancer cells, retain intratumoral heterogeneity, and better reflect the relevant human components of the tumor microenvironment. His models are for more accurate representations of human cancer then traditional cancer cell lines and have the potential to improve the preclinical evaluation of novel anticancer therapies.

In addition, these models may be useful in comparing molecular characteristics or tumor signatures between different subgroups of cancer patients. NSG mice are recommended as immunodeficient mouse model. Due to the severe immunodeficiency of the mice, sterility must be maintained in all experiments.

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Gastric Cancer Patient-derived Xenograft Models Primary Cell Lines Drug Development Tumor Biology Research Tumor Microenvironment NSG Mice Immunodeficient Mouse Model Surgical Procedure Tumor Implantation Vitrification Process DPBS