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JoVE Journal Cancer Research
Caratterizzazione semiautomatica PD-L1 ed enumerazione delle cellule tumorali circolanti da pazienti affetti da cancro del polmone a cellule non piccole mediante immunofluorescenza
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Caratterizzazione semiautomatica PD-L1 ed enumerazione delle cellule tumorali circolanti da pazienti affetti da cancro del polmone a cellule non piccole mediante immunofluorescenza

Article DOI: 10.3791/59873-v • 10:29 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 1,2,3, 1,2, 1,2,3, 4, 4, 5, 1, 3, 6,7, 1,2,3
1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University

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La caratterizzazione delle cellule tumorali circolanti (CTC) è un argomento popolare nella ricerca traslazionale. Questo protocollo descrive un test di immunofluorescenza semi-automatica (IF) per la caratterizzazione PD-L1 e l'enumerazione dei CTC in campioni di pazienti affetti da cancro del polmone non a piccole cellule (NSCLC).

Tags

Ricerca sul cancro problema 150 cancro del polmone cellule tumorali circolanti DNA circolare libero acido di immunofluorescenza CTC dispositivo microfluidico a spirale PD-L1
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