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Journal / Cancer Research / 免疫蛍光による非小細胞肺癌患者からの循環腫瘍細胞の半自動PD-L1特性図と列挙…
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JoVE Journal Cancer Research
免疫蛍光による非小細胞肺癌患者からの循環腫瘍細胞の半自動PD-L1特性図と列挙
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免疫蛍光による非小細胞肺癌患者からの循環腫瘍細胞の半自動PD-L1特性図と列挙

Article DOI: 10.3791/59873-v • 10:29 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 1,2,3, 1,2, 1,2,3, 4, 4, 5, 1, 3, 6,7, 1,2,3
1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University

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循環腫瘍細胞(CTC)の特徴付けは、翻訳研究で一般的な話題です。このプロトコルは、非小細胞肺癌(NSCLC)患者サンプルにおけるCTCのPD-L1特性測定および列挙に関する半自動免疫蛍光(IF)アッセイについて説明する。

Tags

がん研究 問題 150 肺癌 循環腫瘍細胞 循環フリーDNA 免疫蛍光アッセイ CTC スパイラルマイクロ流体装置 PD-L1
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