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Journal / Immunology and Infection / Rensning af ekstracellulære vesikulære præparater …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Rensning af ekstracellulære vesikulære præparater med høj udbytte afstand fra virus
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Rensning af ekstracellulære vesikulære præparater med høj udbytte afstand fra virus

Article DOI: 10.3791/59876-v • 07:15 min • September 12th, 2019
September 12th, 2019 •
*1, *1, 1, 1, 1,2, 3, 4, 5, 1
1Laboratory of Molecular Virology, School of Systems Biology, George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine, Florida International University
* These authors contributed equally

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Denne protokol isolerer ekstracellulære vesikler (EVs) væk fra virions med høj effektivitet og udbytte ved at indarbejde EV nedbør, tæthed gradient ultracentrifugering, og partikel opsamling for at muliggøre en strømlinet arbejdsgang og en reduktion af start volumen krav, hvilket resulterer i reproducerbare præparater til brug i al forskning i EV.

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Immunologi og infektion ekstracellulære vesikler exosome virus oprensning tæthed gradient ultracentrifugering nedbør EV-berigende nanopartikler
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