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Journal / Biology / Forberedelse og udnyttelse af friskisolerede…
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JoVE Journal Biology
Forberedelse og udnyttelse af friskisolerede menneskelige detrusor glatte muskelceller til karakterisering af 9-Phenanthrol-følsomme kation strømme
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Forberedelse og udnyttelse af friskisolerede menneskelige detrusor glatte muskelceller til karakterisering af 9-Phenanthrol-følsomme kation strømme

Article DOI: 10.3791/59884-v • 08:30 min • January 31st, 2020
January 31st, 2020 •
1, 2, 3, 1,3,4
1Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, 2Department of Urology, Medical University of South Carolina, 3Department of Urology, College of Medicine, University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center

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Vi beskriver en metode til fremstilling af enkelt friskisolerede detrusor glatte muskelceller fra humane urinblære prøver anvender en to-trins enzymatisk procedure. De opnåede levedygtige DSM-celler kan studeres ved forskellige enkeltcelleteknikker, herunder den beskrevne amphotericin-B patch-clamp elektrofysiologi for at afsløre fysiologiske og farmakologiske egenskaber.

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Immunologi og infektion urinblære glat muskel detrusor celle isolation enzymatiske menneskelige patch-clamp amphotericin-B
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