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JoVE Journal
Biology

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制备和利用新鲜分离的人类分离器平滑肌细胞,用于9-苯酚敏感阳离子电流的表征
 

制备和利用新鲜分离的人类分离器平滑肌细胞,用于9-苯酚敏感阳离子电流的表征

Article doi: 10.3791/59884
January 31st, 2020

Summary January 31st, 2020

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我们描述了一种从人类膀胱标本中制备单一新分离的分离性肌细胞的方法,采用两步酶程序。获得可行的DSM细胞可以通过各种单细胞技术进行研究,包括所述的amphotericin-B贴片-钳电生理学,以揭示生理和药理特性。

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