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Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Article DOI: 10.3791/59893-v • 10:17 min • August 2nd, 2019
August 2nd, 2019 •
1, 1, 1
1Functional Structure Biology Laboratory, Department of Medical Life Science, Yokohama City University Graduate School of Medical Life Science

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Presynapse formation is a dynamic process including accumulation of synaptic proteins in proper order. In this method, presynapse formation is triggered by beads conjugated with a presynapse organizer protein on axonal sheets of “neuron ball” culture, so that accumulation of synaptic proteins is easy to be analyzed during presynapse formation.

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Presynapse Formation Assay Presynapse Organizer Beads Neuron Ball Culture Presynaptic Proteins Synapse Formation Axon Culture Efficient Analysis Mouse Euthanasia Embryo Dissection Cortices Separation Neuron Ball Culture Trypsinization
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