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Journal / Neuroscience / 使用预突子形成测定使用预突子组织者珠和"神经元球"文化…
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JoVE Journal Neuroscience
使用预突子形成测定使用预突子组织者珠和"神经元球"文化
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Journal (Neuroscience)
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使用预突子形成测定使用预突子组织者珠和"神经元球"文化

Article DOI: 10.3791/59893-v • 10:17 min • August 2nd, 2019
August 2nd, 2019 •
1, 1, 1
1Functional Structure Biology Laboratory, Department of Medical Life Science, Yokohama City University Graduate School of Medical Life Science

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预突生形成是一个动态过程,包括以适当的顺序积累突触蛋白。在这种方法中,前突子形成由与"神经元球"培养的正交片上与前突子组织者蛋白结合的珠子触发,因此在先发形成期间很容易分析突触蛋白的积累。

Tags

神经科学 问题 150 前突生 LRRTM2 本地翻译 脆弱 X 综合征 FMRP Munc18-1 活动区 兴奋性突触
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