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Immunology and Infection

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JoVE Journal Immunology and Infection
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
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Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Article DOI: 10.3791/59894-v • 11:34 min • July 26th, 2019
July 26th, 2019 •
1, 2, 2, 3
1Program in Immunology, Sackler School of Graduate Biomedical Sciences, 2Department of Molecular Virology and Microbiology, Baylor College of Medicine, 3Department of Molecular Biology and Microbiology, Tufts University

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Summary
Automatic Translation

This protocol describes how to induce the differentiation of M cells in human stem cell-derived ileal monolayers and methods to assess their development.

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M Cells Induced Differentiation Human Stem Cell-derived Ileal Enteroid Monolayers Intestinal Homeostasis Immune Defense Transwell Membranes Extracellular Matrix (ECM) Cold Sterile Phosphate Buffered Saline Tissue Culture Incubator Tissue Culture Hood Ileal Enteroids EDTA
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