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Immunology and Infection

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JoVE Journal Immunology and Infection
Diferenciação induzida de pilhas de M cell-like em monolayers Enteroid ileal derivado da pilha de haste humana
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Diferenciação induzida de pilhas de M cell-like em monolayers Enteroid ileal derivado da pilha de haste humana

Article DOI: 10.3791/59894-v • 11:34 min • July 26th, 2019
July 26th, 2019 •
1, 2, 2, 3
1Program in Immunology, Sackler School of Graduate Biomedical Sciences, 2Department of Molecular Virology and Microbiology, Baylor College of Medicine, 3Department of Molecular Biology and Microbiology, Tufts University

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Este protocolo descreve como induzir a diferenciação de pilhas de M em monocamadas ileal derivado da pilha de haste humana e em métodos para avaliar seu desenvolvimento.

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Imunologia e infecção enteroids ileal células-tronco monocamada epitelial polarizada Transpoços células M imunofluorescência células de cálice RANKL GP2 SpiB lgA Lgr5
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