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JoVE Journal Neuroscience
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Ex vivo nervus Slice kultur fra embryonale GFP-uttrykker mus for time-lapse Imaging av nervus nerve utvekst

Article DOI: 10.3791/59911-v • 06:04 min • July 16th, 2019
July 16th, 2019 •
1,2,3, 1,3, 1,3, 1,2,3,4,5,6
1Department of Ophthalmology, Boston Children's Hospital, 2Department of Ophthalmology, Harvard Medical School, 3F.M. Kirby Neurobiology Center, Boston Children's Hospital, 4Department of Neurology, Boston Children's Hospital, 5Department of Neurology, Harvard Medical School, 6Howard Hughes Medical Institute

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En ex vivo Slice analysen lar nervus nerve utvekst å bli avbildet i sanntid. Sektorer genereres ved å bygge inn E 10.5 ISLMN: GFP embryo in agarose, kutting på en vibratome, og vokser i en scene-topp inkubator. Rollen til axon veilednings trasé vurderes ved å legge til hemmere i kultur mediene.

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Nevrovitenskap nervus medfødt skallen dysinnervation uorden axon veiledning øyebevegelser time-lapse Imaging Slice kultur
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